Changes in Color Response of MTT formazan by Zinc Protoporphyrin
نویسندگان
چکیده
منابع مشابه
Reduction of MTT to Purple Formazan by Vitamin E Isomers in the Absence of Cells.
The yellow tetrazolium salt 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) is widely used to determine cell viability in cell proliferation and cytotoxic assays. MTT is reduced by metabolically active cells to form an insoluble purple formazan product that is quantifiable by spectrophotometry. It is the most common and direct assay for cell viability. However, in this pres...
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MTT (3-(4, 5-dimethyl-2-thiazolyl)-2, 5-dihphenyltetrazolium bromide) assay is a widely used method to assess cell viability and proliferation. MTT is readily taken up by cells and enzymatically reduced to formazan, a dark compound which accumulates in cytoplasmic granules. Formazan is later eliminated by the cell by a mechanisms often indicated as exocytosis, that produces characteristic needl...
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ژورنال
عنوان ژورنال: Korean Journal of Food Science and Technology
سال: 2011
ISSN: 0367-6293
DOI: 10.9721/kjfst.2011.43.6.754